FORCIPE

THE RESPONSE OF THE CENTRAL GHRELIN SYSTEM TO THE DEPRIVATION OF CUBS RATS FROM THE MOTHER IN EARLY ONTOGENESIS

  • Nikolay S. Dedanishvili Saint Petersburg State Pediatric Medical University. 2 Lithuania, Saint Petersburg 194100 Russian Federation https://orcid.org/0000-0001-6231-445X
  • Anne-Liza M. Chirkova Saint Petersburg State Pediatric Medical University. 2 Lithuania, Saint Petersburg 194100 Russian Federation
  • Kseniya S. Konopleva Saint Petersburg State Pediatric Medical University. 2 Lithuania, Saint Petersburg 194100 Russian Federation
  • Anastasiya V. Bannova KFU named after V.I. Vernadsky, Order of the Red Banner of Labor Medical Institute named after S.I. Georgievsky. 5/7 Lenin Blvd., Simferopol Republic of Crimea 2905051 Russian Federation
  • Anastasiya E. Matyushevskaya Saint Petersburg State Pediatric Medical University. 2 Lithuania, Saint Petersburg 194100 Russian Federation
  • Andrey V. Kutuzov Saint Petersburg State Pediatric Medical University. 2 Lithuania, Saint Petersburg 194100 Russian Federation
  • Polina A. Petrovskaya Saint Petersburg State Pediatric Medical University. 2 Lithuania, Saint Petersburg 194100 Russian Federation
  • Polina A. Kantor Saint Petersburg State Pediatric Medical University. 2 Lithuania, Saint Petersburg 194100 Russian Federation
  • Maksim S. Oganyan Saint Petersburg State Pediatric Medical University. 2 Lithuania, Saint Petersburg 194100 Russian Federation
Keywords:
материнская депривация выращивание в изоляции грелин maternal deprivation growing up in isolation ghrelin

Abstract

Introduction. Early-life stress can exert profound and long-lasting effects on organism development, manifesting as impaired adaptation, increased anxiety, depressive behavior, and other emotional disturbances. The aim of this study was to investigate the response of the central ghrelin system to maternal deprivation during early ontogeny in Wistar rats. Materials and methods. The experiment involved 40 male Wistar rats (6 litters), which were randomly divided into two groups: control (Con, n=20) and maternal deprivation (MD, n=20). From postnatal day 2 to day 12, pups in the MD group were separated from their mothers for 180 minutes daily and placed in individual plastic containers for ten consecutive days. At the age of 90–100 days (body weight 200–250 g), the animals were sacrificed by decapitation. The hypothalamus, amygdala, and prefrontal cortex were rapidly dissected, immediately frozen in liquid nitrogen, and stored at temperature–80 °C until RT-PCR analysis. Results. No statistically significant differences were found in ghrelin receptor mRNA expression between groups in the hypothalamus or prefrontal cortex. However, in the amygdala, the MD group showed a significant increase in Ghsr1a gene expression compared to the control group. Conclusion. These findings suggest that maternal deprivation predominantly affects the extrahypothalamic regulation of the ghrelin system in the brains of the studied animals.

References

Catani C., Jacob N., Schauer E. et al. Family violence, war, and natural disasters: A study of the effect of extreme stress on children’s mental health in Sri Lanka. BMC Psychiatry. 2008;8(33):1–10.

Lang A.J., Aarons G.A., Gearity J. et al. Direct and Indirect Links Between Childhood Maltreatment, Posttraumatic Stress Disorder, and Women’s Health. Behavioral Medicine. 2008;33:125–136.

Fenoglio K.A., Brunson K.L., Baram T.Z. Hippocampal neuroplasticity induced by early-life stress: functional and molecular aspects. Front. Neuroendocrinol. 2006; 27:180–192.

Бычков Е.Р., Карпова И.В., Цикунов С.Г. и др. Действие острого психического стресса на обмен моноаминов в мезокортикальной и нигростриатной системах головного мозга крыс. Педиатр. 2021; 12(6):35–42.

Cabral A., Portiansky E., Sanchez-Jaramillo E., Zig­man J.M., Perello M. Ghrelin activates hypophysiotropic corticotropin-releasing factor neurons independently of the arcuate nucleus. Psychoneuroendocrinology. 2016;67:27–39. DOI: 10.1016/j.psyneuen.2016.01.027.

Деданишвили Н. С., Пюрвеев С.С., Помигалова А.М. Материнская депривация изменяет экспрессию гена рецептора грелина Ghsr1a в миндалевидном теле у самцов крыс. Актуальные проблемы биомедицины — 2023: материалы XXIX всероссийской конференции молодых учёных с международным участием. 2023:62–64.

Пюрвеев С.С., Брус Т.В., Деданишвили Н.С., Кравцова А.А., Балашов Л.Д., Некрасов М.С. Исследование поведенческой активности у взрослых крыс, подвергшихся стрессовому воздействию в раннем постнатальном периоде. Forcipe. 2022;5(S2):433–434.

Лебедев А.А., Москалев А.Р., Абросимов М.Е. и др. Действие антагониста нейропептида Y BMS193885 на переедание и эмоциональные реакции, вызванные социальной изоляцией у крыс. Обзоры по клинической фармакологии и лекарственной терапии. 2021;19(2):189–202.

Deschaine S.L., Farokhnia M., Gregory-Flores A., Zallar L.J., You Z.B., Sun H., Harvey D.M., Marchet­te R.C.N., Tunstall B.J., Mani B.K., Moose J.E., Lee M.R., Gardner E., Akhlaghi F., Roberto M., Hougland J.L., Zigman J.M., Koob G.F., Vendrusco­lo L.F., Leggio L. A closer look at alcohol-induced changes in the ghrelin system: novel insights from preclinical and clinical data. Addiction biology. 2022;27(1):e13033. DOI: 10.1111/adb.13033.

Deschaine S.L., Leggio L. From “Hunger Hormone” to “It’s Complicated”: Ghrelin Beyond Feeding Control. Physiology (Bethesda, Md.). 2022;37(1):5–15. DOI: 10.1152/physiol.00024.2021.

Heiman M.L., Greenway F L. A healthy gastrointestinal microbiome is dependent on dietary diversity. Mol. Metab. 2016;5:317–320. DOI: 10.1016/j.molmet.2016.02.005.

Jensen M., Ratner C., Rudenko O., Christiansen S.H., Skov L.J., Hundahl C. et al. Anxiolytic-like effects of increased ghrelin receptor signaling in the amygdala. Int. J. Neuropsychopharmacol. 2016;19:pyv123. DOI: 10.1093/ijnp/pyv123.

Пюрвеев С.С., Лебедев А.А., Сексте Э.А. Бычков Е.Р., Деданишвили Н.С., Тагиров Н.С., Шабанов П.Д. Повышение экспрессии мРНК рецептора Грелина в структурах головного мозга детенышей крыс на модели отделения от матери и социальной изоляции. Педиатр. 2023;14(2):49–58. DOI: 10.17816/PED14249-58.

Hedegaard M.A., Holst B. The complex signaling pathways of the ghrelin receptor. Endocrinology. 2020;161:bqaa020.

Прохорычева А.А., Будько А.И., Игнатова О.М., Вечерская Ю.И., Фокин С.А., Пахомова М.А., Васильев А.Г., Трашков А.П. Модели черепно-мозговой травмы: современные подходы, классификация и перспективы исследования. Педиатр. 2024;15(6):49–61. DOI: 10.17816/PED15649-61.

Edwards A., Abizaid A. Driving the need to feed: insight into the collaborative interaction between ghrelin and endocannabinoid systems in modulating brain reward systems. Neurosci. Biobehav. Rev. 2016;66:33–53. DOI: 10.1016/j.neubiorev.2016.03.032.

Шабанов П.Д., Виноградов П.М., Лебедев А.А. и др. Грелиновая система мозга участвует в контроле эмоционально-исследовательского поведения и двигательной активности крыс, выращенных в условиях стресса социальной изоляции Обзоры по клинической фармакологии и лекарственной терапии. 2017;15(4):38–45.

Dos-Santos R.C., Grover H.M., Reis L.C., Fergu­son A.V., Mecawi A.S. Electrophysiological effects of ghrelin in the hypothalamic paraventricular nucleus neurons. Front. Cell. Neurosci. 2018;12:275. DOI: 10.3389/fncel.2018.00275.

Шабанов П.Д., Лебедев А.А., Мещеров Ш.К. Дофамин и подкрепляющие системы мозга. М.-СПб.: Лань; 2002.

Каган В.Е. Аутизм у детей. Л.: Медицина; 1981. EDN: VOVKXZ.

Шабалов Н.П., Иванов Д.О., Цыбулькин Э.К. и др. Неонатология. Том 2. М.: МЕДпресс-информ; 2004. EDN: QLGBMN.

How to Cite
THE RESPONSE OF THE CENTRAL GHRELIN SYSTEM TO THE DEPRIVATION OF CUBS RATS FROM THE MOTHER IN EARLY ONTOGENESIS. (2025). FORCIPE, 8(1), 11-18. https://forcipe.ru/article/view/6631
Issue
Vol. 8 No. 1 (2025)
Section
ORIGINAL PAPERS
Published
2025-09-24

How to Cite

THE RESPONSE OF THE CENTRAL GHRELIN SYSTEM TO THE DEPRIVATION OF CUBS RATS FROM THE MOTHER IN EARLY ONTOGENESIS. (2025). FORCIPE, 8(1), 11-18. https://forcipe.ru/article/view/6631

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