Экспериментальное моделирование аутоиммунного гепатита: КРАТКИЕ СООБЩЕНИЯ

Ксения Алексеевна Михайлова; Полина Андреевна Кантор; Анастасия Евгеньевна Матюшевская; Анастасия Викторовна Баннова

doi:10.56871/

Experimental modeling of autoimmune hepatitis

BRIEF MESSAGES

Ksenia A Mikhaylova Saint Petersburg State Pediatric Medical University
Polina A. Kantor Saint Petersburg State Pediatric Medical University
Anastasia E. Matyushevskaya Saint Petersburg State Pediatric Medical University
Anastasia V. Bannova Order of the Red Banner of Labor Medical Institute named after S.I. Georgievsky V.I. Vernadsky Crimean Federal University

Keywords:

autoimmune hepatitis sodium silicate experimental model rats аутоиммунный гепатит силикат натрия экспериментальная модель крысы

Abstract

Introduction. Autoimmune hepatitis (AIH) is a chronic inflammatory liver disease characterized by the presence of specific autoantibodies and elevated gamma-globulin levels targeting hepatocytes. Currently, it is recognized as a widespread autoimmune disorder with challenges in early diagnosis. The global annual incidence rate is estimated at 1.37 per 100,000 population. Aim — to create an experimental model of autoimmune hepatitis in laboratory animals. Materials and methods. Male Wistar rats (n=5) received an oral solution of sodium silicate once a week for five weeks. After the experiment, biochemical and histological analyses of liver tissue were performed. Results. Compared with the control group, the experimental rats showed significant increases in intracellular enzyme levels: AST (137.6±8.8 U/L) and ALT (35.2±2.2 U/L) (p <0.05), confirming hepatocyte damage. Alkaline phosphatase (ALP) increased significantly (28.9±2.3 U/L), indicating the development of cholestatic syndrome (p <0.05). Total cholesterol (1.9±0.2 mmol/L) and triglycerides (0.7±0.2 mmol/L) were elevated (p <0.001). Total bilirubin rose to 12.5±0.9 µmol/L. Histological examination revealed hepatocyte damage and inflammatory infiltration consisting of lymphocytes and plasma cells. Conclusion. An experimental model of autoimmune hepatitis was successfully established. Characteristic histological and biochemical changes were identified. These findings may serve as a foundation for future research on autoimmune hepatitis.

Author Biography

Ksenia A Mikhaylova, Saint Petersburg State Pediatric Medical University

5th year student.

References

1. Брус Т.В., Васильев А.Г., Трашков А.П. Основные биохимические маркеры при неалкогольной жировой болезни печени (экспериментальное исследование). Патологическая физиология и экспериментальная терапия. 2022;66(1):44–51.

2. Urzua A., Pizarro C., Gajardo A., Poniachik R., Pavez C., Cattaneo M. et al. Autoimmune hepatitis with acute presentation: Clinical, biochemical, and histological features of 126 patients. Can J Gastroenterol Hepatol. 2022;2022:6470847. DOI: 10.1155/2022/6470847.

3. Lamba M., Ngu J.H., Stedman C.A.M. Trends in incidence of autoimmune liver diseases and increasing incidence of autoimmune hepatitis. Clin Gastroenterol Hepatol. 2021;19(3):573–9.e1. DOI: 10.1016/j.cgh.2020.05.061.

4. Брус Т.В., Евграфов В.А. Патофизиология печеночной недостаточности. Педиатр. 2022;13(3):55–64.

5. Шварц В.Я., Ногаллер А.М. Аутоиммунный гепатит. Клиническая медицина. 2013;91(9):57–61.

6. Hahn J.W., Yang H.R., Moon J.S., Chang J.Y., Lee K., Kim G.A. et al. Global incidence and prevalence of autoimmune hepatitis, 1970–2022: A systematic review and meta-analysis. Eclinicalmedicine. 2023;65:102280. DOI: 10.1016/j.eclinm.2023.102280.

7. Grossar L., Raevens S., Van Steenkiste C., Colle I., De Vloo., Orlent H. et al. External validation of the IAIHG autoimmune hepatitis response criteria in a multicentric real-world cohort. JHEP Reports. 2024;6(9):101149. DOI: 10.1016/j.jhepr.2024.101149.

8. Al-Mogairen S.M. et al. Induction of autoimmunity in Brown Norway rats by oral and parenteral administration of sodium silicate. Lupus. 2009;18(5):413–417. DOI: 10.1177/0961203308098192.

9. Mack C.L., Adams D., Assis D.N., Kerkar N., Manns M.P., Mayo M.J. et al. Diagnosis and management of autoimmune hepatitis in adults and children. Hepatology. 2020;72(2):671–722. DOI: 10.1002/hep.31065.

10. Liberal R., Vergani D., Mieli-Vergami G. Update on autoimmune hepatitis. J Clin Trans Hepatol. 2015;3(1):42–52. DOI: 10.14218/JCTH.2014.00032.

11. Ahn S., Jeong S.H., Cho E.J., Lee K., Kim G., Kim H. Comparison of four histological scoring systems for autoimmune hepatitis to improve diagnostic sensitivity. Clin Mol Hepatol. 2024;30(1):37–48. DOI: 10.3350/cmh.2023.0325.

12. Czaja A.J. Diagnosis and management of autoimmune hepatitis: Current status and future directions. Gut Liver. 2016;10(2):177–203. DOI: 10.5009/gnl15352.

13. Terzioli Beretta-Piccoli B., Mieli-Vergani G., Vergani D. Autoimmune hepatitis: Serum autoantibodies in clinical practice. Clin Rev Allergy Immunol. 2022;63(2):124–37. DOI: 10.1007/s12016-021-08888-9.

14. Ивашкин В.Т., Буеверов А.О., Абдулганиева Д.И. Клинические рекомендации по диагностике и лечению аутоиммунного гепатита. М.; 2013.

15. Брус Т.В., Васильев А.Г., Кравцова А.А., Васильева А.В., Брус Ю.С., Баннова А.В. Биомоделирование жировой дистрофии печени смешанной этиологии. Педиатр. 2023;14(5):25–32.

Experimental modeling of autoimmune hepatitis: BRIEF MESSAGES. (2026). FORCIPE, 8(2), 30-35. https://doi.org/10.56871/

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